Reporter

Part:BBa_K1021021:Design

Designed by: Swati Sureka   Group: iGEM13_Cornell   (2013-09-13)


PT7+mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 607
    Illegal AgeI site found at 719
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This reporter does not contain a bacterial RBS and is therefore suited for T7-based expression in eukaryotes.


Source

The promoter originates from the T7 virus and the gene is a highly engineered version of RFP from Discosoma striata.

References